GLP-1 Kit ELISA

N° du produit ABIN2648664

Ce kit ELISA Humain, Souris et Rat GLP-1 est un kit ELISA Colorimetric conçu pour quantifier Humain, Souris et Rat GLP-1.

Aperçu rapide pour GLP-1 Kit ELISA (ABIN2648664)

Antigène: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Épitope: total

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Plasma

Détail du produit GLP-1 Kit ELISA

Sensibilité: 0.6 pmol/L

Attributs du produit: The primary amino acid sequence of GLP-1 peptide is identical among mammalian species, i.e. rat, mouse, pig, human, etc. GLP-1 Total ELISA Assay Kit (enzyme-linked immunoassay kit) is intended quantitative determination of sum value of glucagon-like peptide-1 (7-36) and (9-36) [GLP-1 (7-36)] and (9-36) [GLP-1 (9-36)] in plasma samples. Eagle Biosciences GLP-1 Total ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Protocole: Assay standards, controls and test samples are directly added to wells of a microplate that is coated with streptavidin. Subsequently, a mixture of biotinylated GLP-1 specific antibody and a horseradish peroxidase (HRP)-conjugated GLP-1 specific antibody is added to each well. After the first incubation period, a "sandwich" immunocomplex of "Streptavidin - Biotin-Antibody - GLP-1(7-36)/(9-36) - HRP-conjugated antibody" is formed and attached to the wall of the plate. The unbound HRP-conjugated antibody is removed in a subsequent washing step. For the detection of this immunocomplex, each well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the immunocomplex bound to GLP-1 (7-36)/(9-36) on the wall of the microtiter well is directly proportional to the amount of Total GLP-1 in the sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: GLP-1 (Glucagon-like peptide 1 (GLP-1))

Autre désignation: GLP-1